84-1055-29　Glucose Regulated Protein, NT, 75kD (Grp 75) 200ul　G3057-12

特徴

• Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat inducible .
• Grp75 is actually induced under conditions of low glucose and other nutritional and environmental stresses.
• Grp75 resides primarily in the mitochondrial matrix, where it collaborates with Hsp60 in the re-folding of proteins translocated into this organelle.
• Related forms may also be found in the cytosol or on the surface of the extracellular membrane.
• Other Grp75 functions include its ability to inactivate the tumor suppressor p53.
• Studies have found that Grp75 is over-expressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation.
• Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts.
• And finally, like its E.coli homolog DnaK, GRP75 possesses a cation-dependent ATPase activity considered central to its function as a chaperone.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500 using ECL at 1-2 minute exposure
• Optimum dilutions to be determined by researcher.
• Secondary Antibody:Rabbit IgG (HRP) diluted at 1:100,000
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.