Cytochrome P450 is mainly found in liver, and can metabolize a wide range of compounds such as organic solvents, acetaminophen, dimethylnitrosoamine and aliphatic alcohol, which have certain toxicological effects in humans.
Ethanol-induced cytochrome P450 IIE1 is considered to be one of the major causes of oxidative stress in the liver following ethanol treatment.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000 will yield a positive result with ≥0.2ug of recombinant human cytochrome P450 2E1 using alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody
Optimum dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 20mM phosphate buffer, pH 8.0.
Specificity：Recognizes a band (P450 2E1) in human liver microsomes. Reactive with ≥0.2ug of recombinant human P450 1A2. Only slightly crossreacts with recombinant human P450 1A1 after prolonged incubation. Does not crossreact with recombinant human cytochromes P450 2E1, 3A4 or 2C10.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human P450 2E1 expressed in E. coli
