Applications:Suitable for use in Western Blot and inhibition assays.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:2000 for will yield a positive result with ≥0.2ug of recombinant human cytochrome P450 IA2 using alkaline phosphatase-conjugated anti-goat IgG secondary antibody.
Recognizes a band (P450 1A2) in human liver microsomes
Inhibition: 10mg IgG/nmol P450.
Inhibits ~80% of the ethoxyresorufin O-deethylase activity of human liver microsomesOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：goat
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 20mM Phosphate buffer, pH 8.0
Specificity：Reactive with ≥0.2ug of recombinant human P450 1A2. Only slightly crossreacts with recombinant human P450 1A1after prolonged incubation. Does not crossreact with recombinant human cytochromes P450 2E1, 3A4, or 2C10.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant human P450 1A2 expressed in E. coli
