Phase 1 drug metabolism is catalyzed by two groups of monooxygenases, the cytochrome P450s (CYPs) and the flavin monooxygenases (FMOs).
Both CYPs and FMOs are localized in the endoplasmic reticulum.
Collectively, these enzymes catalyze the oxidation of a very broad range of substrates.
Both classes are dependent on NADPH as a source of electrons, but the two classes exhibit significant differences in mechanism and substrate specificity.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50 dilution will yield a positive result with 0.2ug of recombinant human cytochrome P450 1A1 and/or 1A2 using HRP-conjugated rabbit antigoat antibodies according to the manufacturer's instructions
Optimum dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：goat
Source Antibody：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes both forms in liver microsomes prepared from rabbits or rats that were induced to express multiple forms of P450.
