Hyaluronan binding protein 1 (HABP1), also known as C1qBP/C1qR and p32, is a ubiquitous acidic glycoprotein that functions in spermatogenesis and as a receptor for proinflammatory molecules (1, 2).
HABP1 is synthesized with a 71 amino acid (aa) N-terminal preproprotein and a 208 aa mature region (3).
The 3kD mature mouse HABP1, which contains a MAM33-like sequence, shares 90% and 99% aa sequence identity with human and rat HABP1, respectively.
HABP1 assembles into a doughnut shaped trimer, with negatively charged residues asymmetrically distributed on one face lining the channel of the complex (4).
HABP1 can be cleaved by cell surface MMP-14/MT1-MMP, a protease important in angiogenesis and tumor metastasis (5).
Cell surface HABP1 binds a wide range of extracellular molecules, including hyaluronan, vitronectin, complement component C1q, HMW kininogen, and bacterial and viral proteins (2, 6-9).
Within the cell, HABP1 binds to molecules containing the C1q globular domain, multiple isoforms of PKC, mitochondrial Hrk, the cytoplasmic tails of adrenergic and GABA-A receptors, the mRNA splicing factor ASF/SF2, and the CBF transcription factor (10-16).
Apoptosis and direct phosphorylation by Erk1/2 induces HABP1 translocation to the nucleus (17).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Also Available:H7980-30: Hyaluronic Acid Binding Protein (HABP)H7980-30A: Hyaluronic Acid Binding Protein (HABP) Pab Rb xBoH7980-35: Hyaluronic Acid Binding Protein (HABP) (Biotin)
仕様
Size：1ml
Host：rabbit
Source Antibody：bovine
Grade：Serum
Purity：Neat serum
Form：Supplied as a liquid, serum, before the addition of glycerol to 40%.
Specificity：Recognizes Hyaluronic Acid Binding Protein (HABP). Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Bo Mo Rt
Immunogen：Bovine Hyaluronic Acid Binding Protein (HABP)
