84-1055-11　［取扱停止］CD3 (Early T Cell Marker) (BSA and Azide Free) 250ul　C2254-30X

特徴

• The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR).
• Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition.
• The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage.
• In cortical thymocytes, CD3 is predominantly intracytoplasmic; however, in medullary thymocytes, it appears on the T cell surface.
• CD3 antigen is a highly specific marker for T cells and is present in majority of T cell neoplasms.
• CD3 antigen is expressed on approximately 60–80% of human peripheral blood lymphocytes, 20–40% of splenic lymphocytes, the majority of T-CLL and approximately 70% pf T-All.
• The CD3 complex consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kD.
• Applications:Suitable for use in Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilution:Immunohistochemistry (Formalin/paraffin): 1:10-1:20 for 20 minutes at RT.
• Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes
• Optimal dilutions to be determined by the researcher.
• Positive Control:Tonsil
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.