Chloramphenicol is a chlorine-containing antibiotic produced by Streptomyces venezuelae.
It has a wide spectrum of activity against gram-positive and gram-negative cocci and bacilli (including anaerobes), Rickettsia, Mycoplasma, and Chlamydia.
It inhibits prokaryotic protein synthesis by attaching to the 50S ribosomal subunit.
This inhibits peptidyltransferase, thereby preventing the formation of peptide bonds.
It also inhibits protein synthesis in mitochondria which accounts for its toxic effects that cause aplastic anemia.
Chloramphenicol has other adverse effects such as bone marrow depression, and gray baby syndrome.
It’s use is limited to serious infections such as those where resistance to other antibiotics occurs.
It is still widely used in the treatment of typhoid fever, meningitis and eye infections.
Applications:Suitable for use in ELISA and Immunofluorescence.
Other applications have not been tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：sheep
Grade：Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes Chloramphenicol.
Isotype：IgG
Calc Applications Abbrev：E IF
Immunogen：Aromatic amino derivative of chloramphenicol (prepared by reduction of the nitro group by high-pressure hydrogenation) coupled to KLH, using the diazotization procedure.
