Assayed against 1ug of Rat IgG in a standard capture ELISA using ABTS as a substrate for 30 minutes at RT
Western Blot: 1:1000-1:10,000Optimal working dilutions to be determined by researcher.
Label Molecular Weight:40,000
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months at -20°C after receipt.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
and prior to removing the cap.
仕様
Size：2mg
Host：sheep
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes rat IgG. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-sheep serum, rat IgG and rat serum.
