IL-8, also known as CXCL8, is produced by a range of cells including stimulated monocytes, mast cells, endothelial cells, keratinocytes and fibroblasts.
Human IL-8 promotes neutrophil chemotaxis and degranulation.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5ug/mlWestern Blot: 0.1-0.2ug/mlOptimal working dilutions to be determined by researcher.
Recommended Secondary Reagents:Sheep anti-rabbit IgG labeled with HRP
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a lyophilized powder in PBS. Reconstitute with 100ul sterile ddH2O.
Specificity:Recognizes human Interleukin-8 (IL-8).
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to human IL-8