LIX is a 9.9kD chemokine, expressed by fibroblasts, that is induced by bacterial lipopolysaccharides and is chemotactic for neutrophils.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-2ug/ml using 100ul/well.
The detection limit is 0.2-0.4ng/well of recombinant mouse LIXWestern Blot: 0.1-0.2ug/ml.
The detection limit for recombinant mouse LIX is 1.5-3ng/lane under either reducing or non-reducing conditionssOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from whole serum.
Form：Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes mouse lipopolysaccharide-induced CXC chemokine (LIX), is also known as CXCL5.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to mouse LIX , expressed in E. coli.
