84-1054-24　［取扱停止］Prion Protein, aa79-97 (PrP, CD230) 100ul　P8950-05B

特徴

• CD230 is a 253 amino acid ~27kD GPI anchored membrane protein.
• CD230 is found in large amounts in the brain of patients with transmissible spongiform encephalopathies (TSEs) or prion diseases, fatal infectious neurodegenerative diseases of humans and animals.
• These diseases are biologically unique, as they are believed by some to be transmitted by an infectious agent comprised only of protein, with no nucleic acid component.
• Clinically, these diseases present with motor disturbances and behavioral changes.
• The major pathological changes seen are neuronal loss, vacuolation (spongiform change), proliferation and branching of glial cells, astrocytic proliferation and accumulation of the prion protein PrPSc, which can form amyloid plaques.
• CD230, also known as the prion protein (PrP), exists in two alternate forms; a normal cellular form (PrPc) and a disease-associated form (PrPSc).
• The normal and pathological forms of the prion protein have identical amino acid sequences and differ only in their folded tertiary structure and biochemical properties.
• Applications:Suitable for use in Immunohistochemistry, ELISA, Western Blot.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry (Paraffin): 1:200.
• Does not require protein digestion and does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
• ELISA: 1:10,000-1:30,000Western Blot: 1:2000Optimal working dilutions to be determined by researcher.
• Recommended Positive Control:CJD brain.
• Recommended Secondary Reagents:I1904-28C: IgG, H&L (HRP) Pab Dk xGtI1904-28G: IgG, H&L (HRP) (X-Adsorbed) Pab Dk xGtI1904-28K: IgG, H&L (HRP) Pab Rb xGtI1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab Rb xGtI1904-28V: IgG, Fc (HRP) Pab Rb xGtI1904-29A: IgG, F(ab')2 (HRP) Pab Rb xGt
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.