(Interleukin-15) is a 14kDa immunomodulatory glycoprotein expressed by multiple tissues and cell types, which primarily stimulates T lymphocytes, but is also critical for the development and activation of natural killer cells (NK)
IL-15 shares functional properties with IL-2 and binds with high affinity to the IL-15 receptor (IL-15R), consisting of the IL-15R alpha chain, and also the IL-2Rbeta and common gamma chains used by the IL-2 receptor.
Affects of IL-15 have been linked with cells and tissues unrelated to the immune system, and increased expression of IL-15 has been demonstrated in Rheumatoid Arthritis (RA), ulcerative colitis (UC) Chron’s disease (CD) and lymphoid malignancies, and also conditions affiliated with HIV and HTLV-1 infection.
Applications:Suitable for use in ELISA, Western Blot, Neutralization, Immunohistochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5ug/mlWestern Blot: 0.1-0.2ug/mlNeutralization: 0.15-0.2ug/mlImmunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining paraffin sections.
Sodium citrate buffer, pH 6.0 is recommended
Optimal working dilutions to be determined by researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes human interleukin-15. The antibody will neutralize IL-15 activity in bioassay systems.
Isotype：IgG
Calc Applications Abbrev：E IHC IP N WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human IL-15.
