Recommended Secondary Reagents: Sheep anti Rabbit IgG (Aff.
Pur.): HRP
Applications:Western Blotting: 1-2ug/mlOptimal working dilutions to be determined by researcher.
Western Blotting: Detects a band of approximately 35kD in HepG2 and 3T3 cell lysates.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified IgG was prepared from whole serum by affinity chromatography.
Form:Supplied as a liquid in PBS with 0.02% sodium azide.
Specificity:Recognizes endonuclease G (endoG), a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis endoG i reported to act independently of caspases and may represent a caspase independent apoptotic pathway initiated from the mitochondria. Species Crossreactivity: Human. Expected crossreactivity from sequence similarity mouse, rat.
Isotype:IgG
Calc Applications Abbrev:WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Synthetic peptide GGPRGPGELAKYGLP corresponding to amino acids 55 - 70 of endonuclease G.