PUMA, also known as bbc3, is a pro-apoptotic member of the BCL-2 family.
PUMA is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applictions not tested.
Recommended Dilutions:Western Blot: 2-4ug/ml.
Detects a band at ~23kD in K562 cell lysates
Immunohistochemistry (paraffin): 10ug/ml.
Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended
Optimal working dilutions to be determined by researcher.
Positive Control:Human breast carcinoma
Recommended Secondary Reagents:Sheep anti Rabbit IgG (HRP)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography from whole serum.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes the human p53-upregulated modulator of apoptosis (PUMA).
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide, ARARQEGSSPEPVEG corresponding to the N-terminus of human Bcl-2 binding component 3 (PUMA). Species sequence homology: mouse
