84-1053-57　Vitronectin (VTN, Serum-spreading Factor, S-protein, V75, VN, VNT) 1mg　V2202A

特徴

• Vitronectin (Vn) is a plasma and serum glycoprotein with a normal concentration ranging from 200-400ug/ml.
• It exists in both a 75kD single-chain form and a 65+10kD two-chain form.
• Vn can exist in at least two different conformational forms.
• The majority of Vn found in the circulation is present in the native (“closed”) form.
• In this form, most of the binding sites for other ligands are cryptic.
• The second form of Vn, the denatured (“open,” multimeric) form, is a result of a conformational change in the native protein induced by denaturants such as urea, adsorption onto surfaces, low pH or reduction and alkylation.
• This conformational change leads to exposure of the heparin binding site, formation of disulfide-bonded multimers and rupture of the disulfide bond that links the 10kD light chain to the 65kD heavy chain of the two chain form.
• The liver is the primary site of vitronectin synthesis, however, Vn is also found in platelets, megakaryocytes, monocytes and macrophages.
• Vn plays an important role in a number of physiological and pathophysiological processes.
• It promotes the adhesion and spreading of a wide variety of cell types and is a subcomponent of the soluble SC5b-9 complex of complement where it protects bystander cells from cytolysis.
• Vn also plays an important role in fibrinolysis by stabilizing PAI-1 in its active conformation which otherwise rapidly converts to a latent form (1-3).
• Application:Suitable for use in Immunoelectrophoresis and ELISA.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.