84-1053-47　Tissue Factor 1mg　T5575-04

特徴

• Tissue Factor (TF) is expressed in the plasma membranes of many cell types.
• It is an integral membrane glycoprotein consisting of a single chain molecule of 44kD.
• TF consists of an extracellular domain (residues 1-219), a transmembrane domain (residues 220-242) and the C-terminal intracellular domain (residues 243-263).
• Most abundant in the tissue adventitia, TF becomes exposed to blood at the site of vascular injury.
• The availability of TF is important in initiating coagulation by acting as a receptor for both the zymogen and protease forms of plasma Factor VII (FVII and FVIIa).
• It also mediates the conversion of bound FVII to FVIIa.
• The binding of FVII to TF in the presence of a negatively charged surface such as a phospholipid (or cell surface) promotes the auto activation of FVII by FVIIa.
• The TF-FVIIa complex in the presence of calcium ions proteolytically activates FIX and FX.
• These enzyme products are then capable of activating FVII to FVIIa by feedback amplification.
• The activity of TF-.VIIa activity is regulated by a TFPI (Tissue Factor Pathway Inhibitor), a member of the Kunin superfamily of protease inhibitors.
• TFPI contains three Kunitz domains and is able to bind and inhibit the TF-FVIIa complex in the presence of activated FX and calcium ions.
• Antithrombin has also been reported to inhibit FVIIa activity in the presence of TF and heparin.
• Although a membrane protein, low levels of TF products have been demonstrated in plasma.
• Applications:Suitable for use ELISA and Immunoelectrophoresis.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.