Thrombin (EC3.4.21.5, alpha-thrombin) is the product of proteolytic activation of the zymogen prothrombin.
Human thrombin is a two-chain serine protease with a mass of 37kD.
The active site is located within the heavy chain.
Thrombin has a high specificity for certain arginine bonds in protein substrates.
The primary substrate is fibrinogen which thrombin converts to fibrin through the cleavage of four arginyl-glycyl peptide bonds.
Thrombin is also an important activator of platelets, factor XIII, Protein C and TAFI (Plasma procarboxypeptidase B).
In a positive feedback mechanism, thrombin increases the rate of its own production by activation of factors VIII and V.
The rate of thrombin production is subsequently limited indirectly through the activation of Protein C by thrombin, which then inactivates the activated cofactors VIII and V.
The binding of thrombin to thrombomodulin on the cell surface dramatically alters thrombin’s specificity, increasing it’s activity toward Protein C and TAFI, and decreasing it’s activity toward fibrinogen and activating cofactors VIII and V.
In plasma, thrombin activity is inhibited primarily by antithrombin and to a lesser extent heparin cofactor II.
The rate of inhibition by both of these inhibitors is profoundly increased in the presence of optimal concentrations of heparin.
Other physiological inhibitors of thrombin in the absence of heparin include alpha 2-macroglobulin and alpha1-antitrypsin.
Applications:Suitable for use in ELISA and Immunoelectrophoresis.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Rz ratio (Reinheitszahl, A403/A280):0.36 as determined spectrophotometrically.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：sheep
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in a buffered stabilizer solution, 50% glycerol. Labeled with Horseradish Peroxidase (HRP).
Specificity：Recognizes human thrombin or prothrombin.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Human a-Thrombin inactivated with PPACK.
