84-1053-41　Thrombin 500ug　T5045-04

特徴

• Thrombin (EC3.4.21.5, alpha-thrombin) is the product of proteolytic activation of the zymogen prothrombin.
• Human thrombin is a two-chain serine protease with a mass of 37kD.
• The active site is located within the heavy chain.
• Thrombin has a high specificity for certain arginine bonds in protein substrates.
• The primary substrate is fibrinogen which thrombin converts to fibrin through the cleavage of four arginyl-glycyl peptide bonds.
• Thrombin is also an important activator of platelets, factor XIII, Protein C and TAFI (Plasma procarboxypeptidase B).
• In a positive feedback mechanism, thrombin increases the rate of its own production by activation of factors VIII and V.
• The rate of thrombin production is subsequently limited indirectly through the activation of Protein C by thrombin, which then inactivates the activated cofactors VIII and V.
• The binding of thrombin to thrombomodulin on the cell surface dramatically alters thrombin’s specificity, increasing it’s activity toward Protein C and TAFI, and decreasing it’s activity toward fibrinogen and activating cofactors VIII and V.
• In plasma, thrombin activity is inhibited primarily by antithrombin and to a lesser extent heparin cofactor II.
• The rate of inhibition by both of these inhibitors is profoundly increased in the presence of optimal concentrations of heparin.
• Other physiological inhibitors of thrombin in the absence of heparin include alpha 2-macroglobulin and alpha 1-antitrypsin1-4.
• Applications:Suitable for use in Immunoelectrophoresis and ELISA.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.