Prothrombin (factor II, F.II) is a vitamin K-dependent glycoprotein produced in the liver.
The concentration of prothrombin in plasma is ~100ug/ml (~1.4uM).
Prothrombin is a single chain molecule with a molecular weight of 72kD.
Prothrombin consists of a catalytic domain followed by two kringle structures and an N-terminal domain containing 10 g-carboxy-glutamic acid (gla) residues.
These gla residues allow prothrombin to bind to membranes that contain acidic phospholipids in a calcium dependent manner.
The binding to membranes is required for effective presentation of prothrombin as a substrate for activation by the prothrombinase complex, which consists of activated factor X, activated cofactor V and calcium on phospholipid membrane.
Activation by prothrombinase occurs by sequential cleavage after residue Arg320 then after Arg271 to produce the active protease a-thrombin (37kD) and the byproduct prothrombin fragment 1.2 (35kD).
The product thrombin further cleaves prothrombin fragment 1.2 after residue Arg155 into individual prothrombin fragments 1 and 2.
The activity of a-thrombin in plasma is inhibited primarily by antithrombin and the rate of inhibition is accelerated 1000-fold in the presence of optimal concentrations of heparin.
Other physiological inhibitors of thrombin in the absence of heparin include a2macroglobulin and a1antitrypsin (1-3).
Factor II also plays a role in maintaining vascular integrity during development and postnatal life.
Mutations in Factor II leads to various forms of thrombosis and dysprothrombinemia.
Application:Suitable for use in ELISA and Immunoelectrophoresis (IEP).
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Also Available:P9115-16: Prothrombin Pab Sh xHuP9115-16A: Prothrombin (HRP) Pab Sh xHuP9119A: Prothrombin Pab Sh xHu
仕様
Size：500ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied in 10mM HEPES, pH7.4, 0.15M sodium chloride, 50% glycerol.
Specificity：Specific for human prothrombin as demonstrated by Immunoelectrophoresis. Does not crossreact with prothrombin-deficient plasma.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Human Prothrombin (Factor II) purified from plasma.
