84-1053-22　Protein C Inhibitor (PCI) 500ug　P9102-34

特徴

• Protein C Inhibitor (PCI), also known as Plasminogen Activator Inhibitor 3 (PAI3), is a member of the SERPIN family of proteinase inhibitors.
• It is produced in the liver as a single chain glycoprotein (mass of 57kD) and circulates in plasma at a concentration of 5ug/ml (~90nM).
• PCI is also found in urine in lower concentrations of 250ng/ml (~0.4nM)
• PCI is the primary inhibitor of activated Protein C (APC) in plasma but demonstrates a relatively broad specificity, also inhibiting thrombin, FXa, FXIa, kallikrein, tPA, urokinase, prostate specific antigen, acrosin, chymotrypsin and trypsin.
• The preferred enzyme target for PCI appears to be thrombin and this interaction is increased by more than 100 fold in the presence of thrombomodulin.
• Like ATIII and HCII, the inhibitory activity of PCI towards some of these enzymes is stimulated by high concentrations of heparin (5U/ml) which can accelerate the rate of inactivation as much as 50 fold.
• Enzyme inhibition by PCI occurs through proteolytic cleavage at Arg354-Ser355 and subsequent rapid formation of a stable, inactive 1:1 enzyme-PCI complex.
• Interaction with APC results in an SDS-stable APC-PCI complex of 102kD.
• Applications:Suitable for use in ELISA and Immunoelectrophoresis.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.