Protein C (PC) is a vitamin K-dependent glycoprotein produced in the liver.
The concentration of PC in plasma is ~4ug/ml (~60nM).
A deficiency of Protein C (quantitative or qualitative) is a risk factor for vascular thrombosis.
Protein C is expressed as a two-chain molecule with a molecular weight of 62kD.
The light chain (21kD) of PC consists of two EGF-like domains and an amino-terminal domain containing one hydroxyaspartic acid and 11 gamma-carboxyglutamic acid (gla) residues.
These residues allow PC to bind to membranes that contain acidic phospholipids in a calcium dependent manner.
The heavy chain of PC (41kD) consists of the catalytic domain and an activation peptide.
Activation of Protein C results from cleavage at residue Arg12 in the heavy chain by a complex of thrombin and a cell surface cofactor thrombomodulin.
The activation of PC is associated with the release of a small activation peptide (2-3kD, called Protein C peptide, or PCP) from the N-terminal of the heavy chain.
Activated Protein C (APC) is a serine protease with anticoagulant activity.
APC, in complex with a phospholipid membrane, calcium and the Protein S cofactor, exhibits anticoagulant activity through the proteolytic inactivation of coagulation cofactors Va and VIIIa.
The primary inhibitor of APC activity in plasma is Protein C Inhibitor (PCI, Plasminogen Activator Inhibitor-3, PAI-3) and to a lesser extent by alpha1antitrypsin and alpha2macroglobulin.
The inhibitory activity of PCI is stimulated ~10 fold by heparin.
Applications:Suitable for use in ELISA and Immunoelectrophoresis.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.4, 150mM sodium chloride, 50% glycerol.
