Plasminogen (Pg) is synthesized in the liver and circulates in plasma at a concentration of ~200ug/ml (~2.3uM).
Plasminogen is a single-chain glycoprotein of ~88kD that consists of a catalytic domain followed by five kringle structures.
Within these kringle structures are four low-affinity lysine binding sites and one high-affinity lysine binding site.
It is through these lysine binding sites that plasminogen binds to fibrin and to alpha-2-antiplasmin.
Native plasminogen (glu-plasminogen) exists in two variants that differ in their extent of glycosylation, and each variant has up to six isoelectric forms with respect to sialic acid content, for a total of 12 molecular forms.
Activation of glu-plasminogen by the plasminogen activators urokinase (UPA), or tissue plasminogen activator (tPA) occurs by cleavage after residue Arg560 to produce the two-chain active serine protease plasmin.
In a positive feedback reaction, the plasmin generated cleaves an ~8kD peptide from glu-plasminogen, producing lys77-plasminogen which has a higher affinity for fibrin and when bound is a preferred substrate for plasminogen activators such as urokinase.
Additional activators of plasminogen include kallikrein and activated factor XII.
The primary inhibitor of plasmin in plasma is alpha-2-antiplasmin.
Other physiological inhibitors of plasmin include alpha-2-macroglobulin and antithrombin1-3.
Applications:Suitable for use in ELISA and Immunoelectrophoresis.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.01M HEPES, pH 7.4, 0.15M sodium chloride and 50% glycerol.
Specificity：Recognizes human Plasminogen. IEP against normal plasma gives a single precipitin line. No reaction was obtained with plasminogen- deficient plasma. Plasminogen was detected by ELISA against purified standards
