Intended for use in the neutralization of HCII activity in clot-based assays.
The recommended procedure is to mix 10ul of the neutralizing IgG with 90ul of plasma and allow to incubate at room temperature for 20 minutes before use.
As a control for dilution and IgG addition, in a separate tube add 10ul of the control IgG to another 90ul of the same plasma and also incubate for 20 minutes.
Both plasmas may then be used directly or placed on ice for up to 4 hours.
These conditions are intended for neutralizing the inhibitory activity of HCII in the presence of dermatan sulfate (50ug/ml), providing HCII levels are within the normal range.
Materials Supplied:1) Neutralizing IgG:100ul purified sheep antibody for neutralizing HCII inhibitor activity in plasma.
2) Control IgG:100ul purified sheep IgG from non-immune serum for use as negative control
Both IgGs supplied in a buffer consisting of 20mM M Hepes, 0.15M NaCl, 10mM Na-Citrate, pH 7.4, 0.02% sodium azide.
