Heparin Cofactor II (HCII), also known as heparin cofactor A and dermatan sulfate cofactor, is a single chain glycoprotein produced in the liver and circulates in plasma at concentration of 50-100ug/ml (0.8-1.5uM).
A member of the SERPIN family of proteinase inhibitors, HCII exhibits a relatively narrow specificity and is only known to inhibit the serine proteinases thrombin and chymotrypsin.
The inhibitory activity toward thrombin is accelerated up to 1000-fold in the presence of optimal concentrations of heparin.
Unlike Antithrombin, thrombin inhibition by HCII is also enhanced by dermatan sulfate.
HCII has an apparent molecular weight of 65,600 daltons and interaction with thrombin results in a covalent 1:1 enzyme-inhibitor complex of 102,600 daltons (1-3).
Applications:Suitable for use as capture antibody in ELISA for Heparin Cofactor II and its complexes.
Also suitable for use in Immunodiffusion versus citrated plasma.
Other applications not tested.
Recommended Dilutions: Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.01M HEPES, pH7.4, 0.15M sodium chloride and 50% glycerol.
Specificity：Recognizes human Heparin Cofactor II (HCII). Monospecific when analyzed by immunoelectrophoresis. Only one arc was seen with normal human plasma. No precipitin band was observed with HCII-deficient plasma. Species Crossreactivity: Canine, mouse, porcine, rabbit and rat (Crossrectivity was tested in Immunodiffusion only).
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Human Heparin Cofactor II purified from plasma.
