Fibrinogen is an abundant plasma protein (5-10uM) produced in the liver.
The intact protein has a molecular weight of 340kD and is composed of 3 pairs of disulphide-bound polypeptide chains named Aα, Bβ and γ.
Fibrinogen is a triglobular protein consisting of a central E domain and terminal D domains.
Proteolysis by thrombin results in release of Fibrinopeptide A (FPA, Aα1-16) followed by Fibrinopeptide B (FPB, Bβ1-14) and the fibrin monomers that result polymerize in a half-overlap fashion to form insoluble fibrin fibrils.
The chains of fibrin are referred to as α, β and γ, due to the removal of FPA and FPB.
The polymerised fibrin is subsequently stabilized by the transglutaminase activated Factor XIII that forms amide linkages between γ chains and, to a lesser extent, α chains of the fibrin molecules.
Proteolysis of fibrinogen by plasmin initially liberates C- terminal residues from the Aα chain to produce fragment X (intact D- E-D, which is still clottable).
Fragment X is further degraded to non- clottable fragments Y (D-E) and D.
Fragment Y can be digested into its constituent D and E fragments.
Digestion of non-crosslinked fibrin with plasmin is very similar to the digestion of fibrinogen, which results in production of fragments D and E.
Degradation of crosslinked fibrin by plasmin results in fragment DD (D-Dimer consisting of the D domains of 2 fibrin molecules crosslinked via the γ chains), fragment E (central E domain) as well as DDE in which fragment E is non-covalently associated with DD.
For human crosslinked fibrin, the relative weights of the cleavage fragments produced are: 184kD for fragment DD, 92kD for D, 50kD for E, 1.54kD for FPA and 1.57kD for FPB.
Application:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：sheep
Source Antibody：human
Grade：Purified
Purity：Purified by caprylic acid and ammonium sulfate precipitations and solid-phase adsorption to remove unwanted crossreactivity.
Form：Supplied as a liquid in 0.01M HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol.
Specificity：Recognizes human Fibrinopeptide A.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic fibrinopeptide corresponding to aa1-16 conjugated to carrier.
