Factor XIII (F.XIII, fibrin stabilizing factor) is the proenzyme form of a transamidase that is essential for normal haemostasis and fibrinolysis, wound healing, female fertility and fetal development.
Extracellular F.XIII consists of A subunits (83kD each) which contain the enzyme moiety, and B subunits (76kD each) which act as a carrier protein for the A subunit in circulation.
Both subunits are produced under separate genetic control.
In plasma, F.XIII exists as a non-covalent tetrameric complex (320kD) of two A-subunits and two B-subunits (A2B2).
The concentration of F.XIII tetramer in plasma is ~25ug/ml (~80nM).
An intracellular form of F.XIII is found in platelets, megakaryocytes and monocytes.
This form of F.XIII presents as a dimer of two A-subunits only and has a molecular weight of 160kD.
The importance of these intracellular stores is demonstrated by the observation that platelets can contribute up to half of the F.XIII activity in platelet rich plasma.
The activation of F.XIII involves several steps.
Thrombin cleaves after Arg37 of each A-subunit in the A2B2 tetramer, releasing a 4.5kD activation peptide.
Additional conformational changes induced by the binding of calcium, and by dissociation of the B-subunits from the A-subunit dimer are required to obtain full enzyme activity.
F.XIIIa is a cysteine protease that catalyses the formation of γ-glutamyl-e-lysyl bonds between the γ and α chains of polymerised fibrin molecules.
Other proteins found crosslinked into fibrin clots by F.XIIIa include fibrinogen, a-2antiplasmin, fibronectin, vitronectin and von Willebrand factor
Applications:Suitable for use in Immunoelectrophoresis and ELISA.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Extinction Coefficient (E1% 280):13.4
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：sheep
Source Antibody：human
Grade：Purified
Purity：Purified by caprylic acid and ammonium sulfate precipitations, and solid-phase adsorption to remove unwanted crossreactivity.
Form：Supplied as a liquid in 10mM HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol.
Specificity：Recognizes human Factor XIII as demonstrated by immunoelectrophoresis and ELISA. Species Crossreactivity: rabbit
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Rb
Immunogen：Human Factor XIII (A2B2) purified from plasma
