Factor XII (F.XII, Hageman factor) is a 76kD, single chainglycoprotein produced in the liver.
In plasma, F.XII circulates as a protease zymogen at a concentration of approximately 30ug/ml (400nM).
Upon vascular injury F.XII binds to negatively charged extravascular surfaces such as cartilage and skin, which facilitate activation of the zymogen to the active serine protease.
Cleavage of F.XII by kallikrein after residue Arg353 produces the enzyme alphaF.XIIa, consisting of a 28kD light chain containing the protease domain, and a 52kD heavy chain containing the anionic surface-binding domain.
Substrates for surface bound F.XIIa include the zymogens prekallikrein(PK) and factor XI(F.XI) as well as the procofactor high-molecular weight kininogen (HK).
The activation of these substrates results in positive feedback activation of F.XII.
Further cleavage of alphaF.XIIa by kallikrein produces the 28kD fragment betaF.XIIa (Hageman factor fragment).
BetaF.XIIa has reduced procoagulant activity as it lacks the anionic surface-binding domain, but is capable of fluid-phase activation of PK, factor VII and complement C1.
The activity of F.XIIa in plasma is regulated predominantly by C1-Inhibitor, with relatively minor contributions by alpha2antiplasmin, alpha2macroglobulinand antithrombin, even in the presence of therapeutic levels ofheparin(1-3).
Applications:Suitable for use as detecting antibody in ELISA, Immunoelectrophoresis and other solid phase immunoassays for Factor XII and its activation products.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Rz Ratio (Reinheitszahl, A403/A280):0.43
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：goat
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 50% glycerol. Labeled with Horseradish Peroxidase (HRP).
Specificity：Recognizes human Factor XII at ~76kD.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Activated human Factor XII (F.XII) purified from plasma.
