84-1052-83　Factor XI 1mg　F0018-53A

特徴

• Factor XI (F.XI) is a coagulation protein produced in the liver that circulates in plasma at ~5ug/ml (30nM).
• The mass of F.XI is 160kD as determined by SDS-PAGE under non-reducing conditions and 80kD upon reduction.
• F.XI consists of two identical 80kD subunits linked by disulphide bonds.
• Each subunit consists of a tandem repeat of four apple domains followed by a serine protease catalytic domain.
• Cleavage of F.XI by activated factor XII or thrombin converts each subunit into a two-chain form and generates two active sites per F.XIa molecule.
• The mass of F.XIa is 160kD unreduced, but upon reduction F.XIa migrates as a heavy chain of 50kD and a light chain of 30kD.
• The catalytic site of F.XIa resides in the light chain.
• In plasma, F.XI or F.XIa circulates in non-covalent 1:1 complex with high molecular weight kininogen, which acts as a cofactor in the activation of F.XI by activated factor XII.
• The activity of F.XIa is regulated by platelets and by several proteinase inhibitors including, in order of decreasing importance, C1-inhibitor, alpha-2antiplasmin, alpha-1antitrypsin and antithrombin.
• Heparin has relatively little effect on the rate of inhibition of F.XIa by antithrombin.
• The only known natural substrate for activated F.XI (F.XIa) is factor IX (Christmas factor) and the only cofactor required for this reaction is ionized calcium (1-3).
• Applications:Suitable for use in ELISA, Immunoelectrophoresis and Neutralization.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.