84-1052-76　Factor X (HRP) 200ug　F0018-04

特徴

• Factor X (F.X) is a vitamin K-dependent glycoprotein produced in the liver.
• The concentration of F.X in plasma is ~10ug/ml (~170nM).
• Factor X is expressed as a two-chain molecule with a molecular weight of 59kD.
• The light chain (17kD) of F.X contains a calcium-binding domain consisting of one hydroxyaspartic acid and 11 gamma carboxyglutamic acid (gla) residues.
• These residues allow F.X to bind to membranes that contain acidic phospholipids in a calcium dependent manner.
• This is followed by two EGF-like domains.
• The heavy chain of F.X (42kD) consists of the catalytic domain, carbohydrate and the activation peptide.
• Activation of F.X to the active enzyme (F.Xa) results from cleavage at residue Arg52 in the heavy chain of F.X by a complex of F.IXa, cofactor VIIIa, calcium and negatively charged phospholipid surface (the tenase complex), or by the F.VIIa-tissue factor complex.
• Both activation pathways result in the release of the activation peptide from the N-terminal of the heavy chain.
• The F.Xa generated is a serine protease responsible for the activation of prothrombin to thrombin in the presence of a phospholipid membrane, calcium and cofactor Va.
• The activity of F.Xa in plasma is inhibited by antithrombin (ATIII), alpha1-antitrypsin, alpha2-macroglobulin and tissue factor pathway inhibitor (TFPI).
• The inhibitory activity of ATIII is stimulated ~1000-fold by heparin.
• Applications:Suitable for use in Immunoelectrophoresis and ELISA.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Rz Ratio (Reinheitszahl A403/A280):0.48
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.