Factor IX (FIX, Christmas Factor) is a vitamin K-dependentglycoprotein produced in the liver.
Plasma concentration of FIXis normally around 5ug/ml (87nM) in plasma.
The biologicalimportance of FIX is demonstrated in Haemophilia B(Christmas disease).
This is an X-linked congenital bleeding disease resulting from a quantitative (low activity and low antigen) or qualitative (low activity and normal antigen) defect in FIX function.
In its proenzyme or zymogen form, FIX is a single chain molecule of 55kD.
It contains two EGF-like domains and an amino-terminal domain containing 12-g-carboxy-glutamic acid (Gla) residues.
These Gla residues allow FIX to bind divalent metal ions and participate in calcium-dependent binding interactions.
Applications:Suitable for use as ELISA, Neutralization and other solid phase immunoassays for Factor IX and its activation products.
Other applications not tested.
Recommended Dilutions:ELISA: Suitable for use as the capture antibody
Neutralizing Activity: 166 Bethesda Units/ml IgG against normal plasma
One Bethesda unit/ml is defined as the amount of inhibitor that resulted in 50% residual F.
IX acitivty after 2 hours at 37°C (Kasper CK et al., Thromb Diath Haemorrh 34:869, 1975).
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography
Form：Supplied as a liquid in 10mM HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol.
Specificity：Recognizes human FIX as analyzed by immunoelectrophoresis and ELISA.
