84-1052-67　Factor IX 500ug　F0017-07

特徴

• Factor IX (F.IX, Christmas Factor) is a vitamin K-dependent glycoprotein produced in the liver.
• Plasma concentration of F.IX is normally around 5ug/ml (87nM) in plasma.
• The biological importance of F.IX is demonstrated in Haemophilia B (Christmas disease), an X-linked congenital bleeding disease resulting from a quantitative (low activity and low antigen) or qualitative (low activity and normal antigen) defect in F.IX function.
• In its zymogen form F.IX is a single chain molecule of 55kD.
• It contains two EGF-like domains and an amino-terminal domain containing 12 y-carboxy-glutamic acid (Gla) residues.
• These Gla residues allow F.IX to bind divalent metal ions and participate in calcium-dependent binding interactions.
• The activation of F.IX occurs by limited proteolysis in the presence of calcium by activated factor XI (FXIa) and/or by a complex of VIIa/tissue factor/phospholipid and activated Factor X between residues Arg146- Ala147 and between Arg180-Val181.
• The terminal activated product in either case is F.IXaB, a two-chain enzyme consisting of a heavy chain (28kD), a light chain (18kD) and an activation peptide product of 11kD.
• F.IX can also be cleaved into inactive products by thrombin and by elastase.
• The activity of F.IXaB in plasma is inhibited by antithrombin and this inhibition is accelerated approximately 1000-fold in the presence of optimal concentrations of heparin.
• Applications:Suitable for use in Immunoelectrophoresis and ELISA.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Extinction Coefficient:E1% 280 = 13.4
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.