Factor VIII is a large glycoprotein (320kD) that circulates in plasma.
Synthesized in the liver, the majority of Factor VIII is cleaved during expression, resulting in a heterogeneous mixture of partially cleaved forms of F.VIII.
FVIII is a pro-factor that is activated through limited proteolysis by thrombin.
Applications:Suitable for use in ELISA and Immunoelectrophoresis.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Rz ratio (Reinheitszahl, A403/A280):0.42
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：sheep
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in a buffered stabilizer solution, 50% glycerol. No preservatives are added. Labeled with Horseradish Peroxidase (HRP).
Specificity：Recognizes human F.VIII as demonstrated by immunoelectrophoresis and ELISA. No reactivity is observed with von Willebrand Factor.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Human Factor VIII:C (F.VIII) purified from F.VIII concentrate.
