Factor VII (F.VII) is a vitamin K-dependent glycoprotein produced in the liver.
Plasma concentration of F.VII is normally ~0.5ug/ml (10nM) in plasma.
A deficiency of F.VII is associated with bleeding in a clinical pattern similar to haemo- philia, but is inherited as an autosomal recessive trait.
The deficiency can be characterized by a quantitative (low activity and low antigen) or a qualitative (low activity and normal antigen) defect in F.VII function.
In its zymogen form F.VII is a single chain molecule of ~50kD.
It contains two EGF-like domains and an amino-terminal domain containing 10 gamma-carboxyglutamic acid (Gla) residues.
These Gla residues allow F.VII to bind divalent metal ions and participate in calcium-dependent binding interactions.
F.VII and activated F.VII (F.VIIa) bind to tissue factor exposed at the site of vascular injury.
F.IXa, F.Xa or F.VIIa rapidly activate tissue factor-bound F.VII to F.VIIa in the presence of calcium and phospholipid.
Thrombin and F.XIIa are able to activate F.VII in the fluid phase in the absence of cofactors.
The activation of the single chain zymogen F.VII occurs by proteolysis after residue Arg152, resulting in a two-chain active serine protease consisting of a 30kD heavy chain and an 18kD light chain.
In complex with tissue factor, phospholipid and calcium, F.VIIa is able to activate F.X and F.IX.
Free F.VIIa in plasma is remarkably stable, but the activity of F.VIIa/TF complex is regulated by Tissue Factor Pathway Inhibitor (TFPI) in the presence of F.Xa, and also by Antithrombin (ATIII) in the presence of heparin.
Applications:Suitable for use in ELISA and Immunoelectrophoresis.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Rz Ratio (Reinheitszahl A403/A280):0.5
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：sheep
Source Antibody：human
Grade：Purified
Purity：Purified.
Form：Supplied as a liquid in a stabilizer protein solution, 50% glycerol. Labeled with Horseradish peroxidase (HRP).
Specificity：Recognizes human Factor VII. No reaction was observed against Factor VII-deficient plasma by immunoelectrophoresis.
