Factor VII (F.VII, also known as Stable Factor and Proconvertin) is a vitamin K-dependent glycoprotein produced in the liver.
Plasma concentration of F.VII is normally ~0.5ug/ml (10nM) in plasma.
A deficiency of F.VII is associated with bleeding in a clinical pattern similar to haemophilia, but is inherited as an autosomal recessive trait.
The deficiency can be characterized by a quantitative (low activity and low antigen) or a qualitative (low activity and normal antigen) defect in F.VII function.
In its zymogen form F.VII is a single chain molecule of ~50kD.
It contains two EGF-like domains and an amino-terminal domain containing 10 g-carboxyglutamic acid (Gla) residues.
These Gla residues allow F.VII to bind divalent metal ions and participate in calcium-dependent binding interactions.
F.VII and activated F.VII (F.VIIa) bind to tissue factor exposed at the site of vascular injury.
F.IXa, F.Xa or F.VIIa rapidly activate tissue factorbound F.VII to F.VIIa in the presence of calcium and phospholipid.
Thrombin and F.XIIa are able to activate F.VII in the fluid phase in the absence of cofactors.
The activation of the single chain zymogen F.VII occurs by proteolysis after residue Arg152, resulting in a two chain active serine protease consisting of a 30kD heavy chain and an 18kD light chain.
In complex with tissue factor, phospholipids and calcium, F.VIIa is able to activate F.X and F.IX.
Free F.VIIa in plasma is remarkably stable, but the activity of F.VIIa/TF complex is regulated by Tissue Factor Pathway Inhibitor (TFPI) in the presence of F.Xa, and also by Antithrombin (ATIII) in the presence of heparin1-3.
Application:Suitable for use in ELISA and Immunoelectrophoresis.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10mg
Host：sheep
Source Antibody：human
Grade：Purified
Purity：Purified by caprylic acid and ammonium sulfate precipitations, and solid -phase adsorption to remove unwanted crossreactivity.
Form：Supplied as a liquid in 10mM HEPES, pH 7.4, 150mM sodium chloride, 50% glycerol. No preservatives are added.
Specificity：Recognizes human Factor VII as demonstrated by immunoelectrophoresis and ELISA.
