Elastase is a serine protease which exhibits endopeptidase activity on many proteins, its principal substrate, however, is elastin and it is unique among proteases in its ability to hydrolyse native elastin.
It is present in the pancreas, leukocytes and serum.
Elastase consists of a single polypeptide chain with a molecular weight of 29.5kD.
Applications:Suitable for use in ELISA and Immunoelectrophoresis.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：sheep
Source Antibody：human
Grade：Purified
Purity：Purified.
Form：Supplied as a liquid in 10mM HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol.
Specificity：Recognizes human Neutrophil Elastase. Monospecific when analyzed by immunoelectrophoresis. No reaction was seen with normal human plasma and only one band was observed in plasma to which active-site blocked elastase had been added. Identity was verified using purified elastase.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Neutrophil elastase (active-site blocked) prepared from human sputum.
