Apolipoprotein-H, also known as b2-Glycoprotein I (b2GPI), is a plasma glycoprotein that circulates at a concentration of 200ug/ml (4uM).
Synthesized in the liver, b2GPI is a single chain molecule of 48kD, consisting of five repeating internally disulfide-bonded structures referred to as sushi domains.
Relative to other glycoproteins, b2GPI has an unusually high content of cysteine (6.2%), proline (8.3%) and carbohydrate (19%).
Almost half the circulating b2GPI in plasma is associated with lipoproteins of all major fractions.
b2GPI has been demonstrated to bind negatively charged phospholipids, heparin and platelets.
Although the precise function(s) are as yet unknown, b2GPI has been demonstrated to interfere with blood coagulation by competitively binding to negatively charged phospholipid surfaces exposed during cell activation or damage.
Recent evidence also implicates b2GPI as a cofactor recognized by anti-phospholipid antibodies present in some autoimmune disorders such as systemic lupus erythematosus (SLE).
Applications:Suitable for use in ELISA and Immunoelectrophoresis.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Extinction Coefficient (E1% 280):13.4
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol.
Specificity：Recognizes human Apolipoprotein H. Species Crossreactivity: porcine, rat, canine, mouse and rabbit
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Ca Hu Mo Po Rb Rt
Immunogen：Human Apolipoprotein H purified from plasma.
