a1-Antitrypsin (a1-AT) is a single polypeptide chain, a typical member of the serine family (serine protease inhibitors), inhibiting the enzyme trypsin.
a1-AT is a glycoprotein synthesized in the liver, and is genetically polymorphic due to the presence of over 20 alleles.
Individuals appropriately homozygous are deficient in a1-trypsin and are predisposed to pulmonary emphysema and juvenile hepatic cirrhosis because of alterations in the amino acid and sialic acid components of the glycoprotein.
The molecular mass of a1-AT is ~52kD.
Applications:Suitable for use in ELISA and Immunoelectrophoresis.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Rz Ratio:Reinheitszahl; A403/A280 = 0.39
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied in buffered stabilizer, 50% glycerol. No preservatives added. Labeled with Horseradish peroxidase (HRP).
Specificity：Recognizes human alpha1-Antitrypsin.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Human alpha1-Antitrypsin (a1-AT) purified from plasma.
