84-1052-43　a1-Antitrypsin (AAT, alpha1 Proteinase Inhibitor, alpha1 PI) 500ug　A2298-27A

特徴

• a1Antitrypsin (a1AT), also known as a1Proteinase inhibitor (a1PI), is the most abundant protease inhibitor in blood and a member of the SERPIN family of proteinase inhibitors.
• Serum levels are typically 1.3mg/ml (2.5uM).
• a1AT is an acute phase protein and concentrations can rise four-fold during inflammatory episodes or tissue injury.
• Low levels in circulation have been associated with pulmonary disease such as emphysema.
• a1AT is a single chain molecule (52kD) that is produced primarily in the liver and to a lesser extent by blood monocytes and intestinal epithelium.
• Based on association rates, the primary target enzyme for a1AT is believed to be neutrophil elastase.
• a1AT is a broad-spectrum inhibitor for many serine proteinases.
• The main role of a1AT in vivo is likely that of a “backup” inhibitor and proteinase scavenger in fluids and tissues.
• Although the association rates of a1AT with other enzymes are lower, the high concentration in plasma makes it an important inhibitor of activated Protein C, activated F.XI, thrombin and plasmin.
• Enzyme inhibition by a1AT occurs through proteolytic cleavage between Met358 and Ser359, which induces a conformational change in a1AT locking the enzyme into a stable, inactive 1:1 enzyme-inhibitor complex.
• Application:Suitable for use in Immunoelectrophoresis and ELISA.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.