84-1052-41　Antithrombin III (ATIII) (HRP) 100ug　A2298-18

特徴

• Antithrombin, also known as Antithrombin III (ATIII), is a member of the SERPIN family of proteinase inhibitors and the primary inhibitor of thrombin in plasma.
• It is produced in the liver and circulates in plasma at ~200ug/ml (~3.5uM).
• Antithrombin inhibits a broad spectrum of serine proteases including thrombin, activated forms of factor X, factor IX, factor XI, factor XII, as well as kallikrein, plasmin and urokinase.
• Enzyme inhibition by antithrombin occurs through proteolytic cleavage at Arg385-Ser386 and subsequent rapid formation of a stable, inactive 1:1 enzyme-antithrombin complex.
• Heparin has a profound accelerating effect on the inhibitory activity of antithrombin towards some enzymes.
• For example, the rate of inhibition of thrombin and activated factor X is increased 1000-fold in the presence of optimal concentrations of heparin, whereas heparin has relatively little effect on the inhibition rate of activated factor XI, activated factor XII and kallikrein.
• Antithrombin is a single chain molecule with a molecular weight of 59kD.
• Interaction with thrombin results in an SDS-stable thrombin-antithrombin complex of 96kD.
• Applications:Suitable for use in ELISA and Immunoelectrophoresis.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Rz Ratio (Reinheitszahl, A403/A280):0.422 as determined spectrophotomerically.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer
• Labeled with horseradish peroxidase (HRP).
• Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.