Members in the TNF superfamily regulate immune responses and induce apoptosis.
A novel member in the TNF family was recently identified by several groups and designated BAFF, BLyS, TALL-1, THANK, and zTNF4.
BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion.
Two receptors, TACI and BCMA, for BAFF were originally identified.
A third receptor was identified recently and designated BAFF-R and BR3 for BLyS receptor 3.
Unlike BCMA and TACI, which bind to BAFF and April, BAFF-R/BR3 is specific for BAFF and plays a predominant role in BAFF induced B cell development and survival.
BAFF and its receptors are involved in B cell associated autoimmune diseases, and activate NF-£eB and c-jun N-terminal kinase.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 5ug/mlImmunohistochemistry (paraffin): 5ug/mlImmunofluorescence: 20ug/ml Optimal dilutions to be determined by the researcher.
Blocking peptide:B0003-68A
Positive Control:Mouse spleen tissue lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human BAFF-R. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the C-terminus of human BAFF-R (aa169-183, NH2-GSTELVTTKTAGPEQ-COOH) (6). The peptide sequence is identical between human and mouse origin (6).
