Thrombopoietin receptor (Tpo R), also known as myeloproliferative leukemia protein (c-mpl), is a 95kD type I transmembrane protein that is a member of the type I cytokine receptor family within the hematopoietin/cytokine receptor superfamily.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 0.25-1ug/ml detects TPO receptor in RIPA lysates from DAMI cells.
DAMI cell lysate was resolved byelectrophoresis, transferred to PVDF and probed with T5050-50 (0.5ug/ml).
Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system
Immunohistochemistry: Stains megakaryocytes in bone marrow biopsy sections
Immunoprecipitation: 5ug immunoprecipitates TPO receptor from 500ug of DAMI RIPA lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes glycosylated and unglycosylated human TPO receptor/c-Mpl, Mr 70kD. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa615-628, C-ANHSYLPLSYWQQP, of the human Thrombopoietin Receptor, conjugated to KLH. Species Sequence Homology: mouse, 100%
