Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2mg/ml detects STAT5B (doublet of 90kD and 92kD) inhuman A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure
Immunoprecipitation: 4ug immunoprecipitates STAT5B from A431 carcinoma cells and a previous lot immunoprecipitated STAT5B from human Raji cells.
A RIPA lysate of human A431 cells (500ug protein) was incubated with 4ug of anti-STAT 5B Immunecomplexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B.
Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection.
Positive Antigen Control:mouse 3T3 cell lysate.
Add 2.5ul of 2-mercaptoethanol per 100ul of lysate and boil for 5 minutes to reduce the preparation.
Load 20ug of reduced lysate per lane for minigels.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Suppied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity：Recognizes the 90-92kD forms of human STAT5B protein. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo
Immunogen：aa774-787 of human STAT5B (CGRPMDSQWIPHAQS)
