84-1052-13 SOS1 (Son of Sevenless Homolog 1, SOS-1) 200ug S5360-18
特徴
- Applications:Suitable for use in Western Blot and Immunoprecipitation.
- Other applications not tested.
- Recommended Dilutions:Western Blot: 0.5-1ug/ml Immunoprecipitation: 5ug Optimal dilutions to be determined by the researcher.
- Positive Control Included:F4209: Fibroblast Cell, 3T3/A31.
- Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
- Load 20ug of reduced lysate per lane for minigels.
- Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRbI1904-46Q: IgG, H&L (HRP) Pab Gt xRb
- Storage and Stability:May be stored at 4°C for short-term only.
- Aliquot to avoid repeated freezing and thawing.
- Store at -20°C.
- Aliquots are stable for 12 months after receipt.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
- Size：200ug
- Host：rabbit
- Source Antibody：mouse
- Grade：Affinity Purified
- Purity：Purified by Protein A affinity chromatography.
- Form：Supplied as a liquid in 0.1M Tris, pH 8.0, 0.15M sodium chloride, 0.1mM EDTA, 0.05% sodium azide, 30% glycerol.
- Specificity：Recognizes mouse SOS1, Mr 175kD. Species Crossreactivity: human.
- Isotype：IgG
- Calc Applications Abbrev：IP WB
- Calc Crossreactivity：Hu Mo
- Immunogen：Synthetic peptide corresponding to aa1243-1258, KKSDHGNAFFPNSPSP of murine Son of Sevenless Homolog 1 Protein (KLH).
- Shelf Life：1year
- Swiss Prot Number：Q62245, Q07889
- EU Commodity Code：30021010
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