Type 2 protein phosphatases are serine/threonine phosphatases which can preferentially dephosphorylate the a subunit of phosphorylase kinase and are insensitive to inhibitor 2 treatment.
Protein phosphatase 2A (PP2A) can be distinguished from PP2B and PP2C which require Ca 2+ and Mg 2+, respectively, by its partial activity in the absence of divalent cations.
PP2A activity is potently inhibited by treatment with okadaic acid and shows much stronger activity toward histone phosphorylated by Protein Kinase C and casein phosphorylated by Protein Kinase A than other protein phosphatases.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 detected PP2A in RIPA lysates from mouse brain, heart, testes and spleen homogenates
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M sodium chloride, 5mg/ml BSA, 0.05% sodium azide.
Specificity：Recognizes human PP2A catalytic subunit, Mr 36kD. Species Crossreactivity: mouse, rat, bovine. Wide range of species Crossreactivity expected due to highly conserved immunogen sequence.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa290-304 (DPAPRRGEPHVTRRT) of human PP2A, KLH-conjugated.
