Applications:Suitable for use in Western Blot, Dot Blot and ChIP.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:5000-1:10,000 to detect trimethylated-Histone H3 (Lys4) in HeLa nuclear acid extracts
Dot Blot: 1:10,000 Optimal dilutions to be determined by the researcher.
Positive Control:HeLa nuclear extracts
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human Histone H3 containing trimethyl-lysine and to a lesser extent, dimethyl-lysine 4. Mr ~17kD. Broad species Crossreactivity is expected. Specificity was confirmed by the ability of 1uM of the immunizing peptide to completely abolish detection of Histone H3 in immunoblot analysisof HeLa acid extracts. Signal reduction was detected with preincubation with peptide containing dimethyl-lysine 4.
Isotype：IgG
Calc Applications Abbrev：ChIP DB WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues surrounding and including trimethlyated Lys 4 of Histone H3.
