Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:10,000 detected acetyl-Histone H3 (Lys27) in acid extracts from sodium butyrate treated HeLa cellsImmunoprecipitation: Immunoprecipitates chromatin from wild type yeast, compared to yeast containing a Histone H3 K27R substitution
Optimal dilutions to be determined by the researcher.
Positive Control:Acid extracted proteins from HeLa cells treated with sodium butyrate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes acetyl-Histone H3 (Lys27), Mr ~17kD. Species Crossreactivity: Yeast and human. Broad species crossreactivity is expected.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Ye
Immunogen：Ovalbumin-conjugated, synthetic peptide (SKAARAcKSAPST-C) corresponding to aa22-32 of yeast Histone H3 acetylated on lysine 27, with a C-terminal cysteine added for conjugation purposes.
