Applications:Suitable for use in Western Blot, Immunocytochemistry, Dot Blot, ChIP and Peptide Inhibition Assay.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000 Immunocytochemistry: 1:500Peptide Inhibition Analysis: 1:500 was blocked by phospho-Histone H3 (Ser28) peptides, but not non-specific peptides
Optimal dilutions to be determined by the researcher.
Positive Control:HeLa acid extract
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes Histone H3 phosphorylated at Ser28. Species Crossreactivity: mouse and human. Has broad species reactivity due to sequence conservation.
Isotype：IgG
Calc Applications Abbrev：ChIP DB IC WB
Calc Crossreactivity：Hu Mo
Immunogen：Linear peptide corresponding to Histone H3 phosphorylated at Ser28 (KLH).
