Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.
Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4).
The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
This gene is intronless and encodes a member of the histone H3 family.
Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Applications:Suitable for use in Western Blot, ChIP and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000-1:5000ChIP: 1ug/mg of total cell protein (~10e7 cells)Optimal dilutions to be determined by the researcher.
Positive Control:Acid-extracted proteins from serum-starved 10T1/2 cells.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 5mg/ml BSA, before the addition of glycerol to 30%.
Specificity：Recognizes human Histone H3 phosphorylated at serine 10 and acetylated at lysine 14, Mr 17kD. An unidentified protein is also seen in some cells lines, Mr 40kD. Species Crossreactivity: mouse; broad species Crossreactivity expected.
Isotype：IgG
Calc Applications Abbrev：ChIP IC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa7-20 of human histone H3 (ARK[pS]TGGAcKAPRKQL-C), KLH-conjugated.
