Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-0.5ug/ml detects CoREST in RIPA lysates from A431, 3T3/A31, Jurkat, and Hela cells.
Reported to be detected in RIPA lysates from HEK 293, L6, PC12, C6 cells and liver, muscle, heart and brain tissue
Immunoprecipitation: 2.5ug immunoprecipitates CoREST from 250ug of A431 RIPA lysate.
Immunocytochemistry: Positive immunostaining for CoREST in cells fixed with 4% paraformaldehyde pH 7.3 for 10-15 minutesImmunohistochemistry: Reported to detect CoREST in tissue sections.
Included Positive Control:A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer.
Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
Load 20ug of reduced lysate per lane for minigels.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human CoREST, Mr ~66kD. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：ChIP IC IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：GST fusion protein corresponding to residues 109-293 of human CoREST.
