Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP-and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1).
It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3).
Three phosphorylation sites, Ser157, Ser239 and Thr278, have been identified.
Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4).
Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5).
Phosphorylation at Ser239 of VASP is a useful tool to monitor PKG activation and signaling (6,7).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human VASP phosphorylated at Ser157. May crossreact with the phosphorylated VASP homologue Mena. Species crossreactivity: mouse, rat, monkey and guinea pig.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Gp Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser157 of human VASP.
